Preparation of products in crude purification and column purification
Accurately prepare reagents and buffers used for protein purification by yourself.
Appropriately lyse the cultured bacteria and perform crude purification of the target proteins with the support of manager/senior members.
Analyze the yield, purity, and concentration of the target protein in target samples collected from the crude and column purification processes and accurately record the results.
Report the progress of purification and the results of purification analysis to manager/senior members in a timely manner.
Perform the work in compliance with the schedule and requirements as directed by manager/senior members.
Preparation of enzyme cocktails
Prepare, dispense, and store enzyme cocktails using purified protein accurately and appropriately with the support of manager/senior members. Also, accurately report to manager/senior members the inventory of stored enzyme cocktails and of lots.
Troubleshooting
Recognize trouble in the result of purification and report it to manager/senior members and team members.
Discuss the cause of the trouble with team members.
Execute solutions to troubles with the support of manager/senior members or experienced technical personnel.
Documentation and management/recording in manufacturing
Understand and comply with company regulations and quality control regulations (QMS/ISO9001) related to manufacturing.
Prepare accurate procedure manuals with the support of manager/senior members using the company's templates. Also, work autonomously in accordance with the procedure manuals.
Accurately record necessary information (numerical values, comments, etc.) obtained in the course of work, and autonomously create a record in accordance with the procedure manuals.
Summarize the data obtained in the work in a concise manner in accordance with the purpose of the work. Also, provide a discussion of what you have observed and noticed based on the data.
Store and update procedures, records, raw data, data summaries, and discussions in an appropriate storage locations, and share them with the team as appropriate.
Store reagents, culture media, samples, deliverables, etc. prepared in the work in an appropriate location. Conduct periodic inventories with the support of manager/senior members and check against this management list. In adition, share this management information with the team in a timely manner.
Management of laboratory environment (if applicable)
Carry out inventory management and stocktaking of reagents, consumables, and raw materials with the support of manager/senior members and secure any shortages in a timely manner in coroperation with the supply chain.
Implement inspection and maintenance of equipment and instruments autonomously in accordance with internal regulations.
Support for other manufacturing processes
Provide support for work in manufacturing processes outside of the area of responsibility with support of manager/senior members.
Qualifications
Education
Undergraduate degree or higher in the field of biology, science and engineering, or agriculture, or in a comparable field
Personal characteristics and work experience
At least 2 years of experience in experimentation and research
Ability to cooperate, listen well, and actively discuss team issues and their solutions
Cooperative in laboratory operations outside of experimental work (e.g., safety initiatives)
Languages
Japanese Language Proficiency: Japanese Language Proficiency Test N2 or higher
English proficiency: Ability to understand English literature and experimental protocols, and to read and write experimental notes in English. Daily conversational level of English is desirable
Experimental skills
Acquisition of basic knowledge of how to handle proteins
Acquisition of basic FPLC methods as wll as of column preparation and handling.
Experience in expression and purification of recombinant proteins using E. coli, utilizing a variety of types and methods
Knowledge and experience with general molecular biological techniques
Knowledge of and experience in handling common chemicals
This position is site-based, requiring you to be at Moderna’s site full-time. This position is not eligible for remote work.
